An investigation of alternative phosphorylation sites in arsR in Helicobacter pylori(part2)

Since the start of this semester, I’ve performed qRT-PCR for several times. qRT-PCR with taqman probes enables us to know the relative concentrations of mRNAs from the cells cultured under acidic (in my case pH5) and neutral(pH7) conditions. This technique requires a lot of patience and great attention to details. Although at the beginning, the error bars for the result bar chart were very big, indicating potential technical problems, I’m getting better results overtime. Practice makes perfect!

From the preliminary data I got, the mutant I created showed repression of sabA transcription consistently. This is different from my original hypothesis that the new mutant will lose its ability to regulate sabA. It’s very interesting that arsR seems to be constitutively activated now. However, its still too early to draw any conclusion. I need to repeat this experiment for several times and perform it with other genes before I reach this conclusion.